Effectiveness, Function, and Preparation Method of erythritol

With the increasing level of people's living standards, the consumption market of sweet food is getting larger and larger. As a result, obesity and diabetes problems have become more and more serious. To solve this problem, food manufacturers have brainstormed different sugar substitutes and one of them is erythritol.

The Efficacy of Xylitol

Xylitol is widely found in nature, like in fungi (lichens and mushrooms), melons, grapes, pears, and is sometimes found in human fluids. According to information on Chemicalbook.com, erythritol is also known as red silk saccharin or Chitosan, a polyalcohol which mainly comes from the glucose fermentation of cornstarch. Compared with ordinary sugar, it generates fewer calories and is much sweeter, with a sweetness of about 70 and only 0.24 calories per gram, respectively. It is an ideal low-calorie sweetener.

The Functions of Xylitol

Due to its low melting point, low hygroscopicity, low calories, high heat absorption during dissolution, high stability towards acid and heat, erythritol is widely used in the food processing industry. Xylitol can be used in baking products, maintaining the taste while preventing moisture. It can even add a unique refreshing taste to biscuits. When added to beverages, erythritol can enhance the taste, while its antioxidant property can protect vitamins. Adding erythritol to candy or chocolate can improve the quality and taste. For example, chocolate that utilizes erythritol can be operated at 80-degrees Celsius due to its good heat stability, low hygroscopicity, and can reduce 34% of calories, resulting in a refreshing taste and non-cariogenic characteristic.

The anti-cavity characteristic of erythritol is a hot topic in its application in the medical industry. The mixture of erythritol and milk can inhibit the growth of mutans streptococci in the biofilm, promote the remineralization of demineralized enamel and help prevent cavities. Xylitol can inhibit Porphyromonas gingivalis and reduce its ability to adhere to the surface of teeth and bones, which is a new approach to prevent and treat gingivitis. The antioxidant property of erythritol can reduce the oxidative emergency damage in the body. Animal experiments have shown that the antioxidant capacity of erythritol can protect the blood vessels that are damaged by high blood sugar in diabetic rats and reduce the risk of heart disease.

Production of Xylitol Fermentation Fluid

1. Choose Moniliella acetoabutans as the starting culture for fermentation, using Moniliella acetoabutans ATCC 18455 as an example in this method.

2. First, sterilize the shake flask medium and then access the Moniliella acetoabutans culture with a shaker at 30 degrees Celsius temperature for 24 hours. The shake flask medium formula is:

① Glucose 200g/L;

② Corn gluten meal powder 3g/L;

③ Urea 5g/L.

3. Add potassium dihydrogen phosphate 10g/L and magnesium sulfate 5g/L to the broken rice sugar liquid in the substrate storage tank, and then pump the second-level seed into the tank after 15 minutes of sterilization at 120 degrees Celsius. After cooling to 30 degrees Celsius, the first-level seed is inoculated with 5% of the culture solution volume. Filter-sterilized sterilized air is introduced into the fermenter with a tank pressure of 0.5 megapascals, a ventilation rate of 0.5 cubic meters per minute, a fermentation temperature of 30 degrees Celsius, and a speed of 200 revolutions per minute. Fermentation is conducted for 24 hours. After examining various indicators such as the seed species mirror and OD value, you get the second-level seed solution that meets the standards.

4. Add potassium dihydrogen phosphate 10g/L, manganese sulfate 5g/L, magnesium sulfate 5g/L, and 0.05% Tween 80 to the broken rice sugar liquid in the fermentation medium storage tank. After 15 minutes of sterilization at 120 degrees Celsius, pump it into the fermenter, add the second-level seed at 10% of the culture solution volume, and keep the temperature in the tank at 28 degrees Celsius for fermentation. Filter-sterilized sterilized air is introduced into the fermenter with a tank pressure of 0.05 megapascals, a ventilation rate of 1 cubic meter per minute, a speed of 400 revolutions per minute, and fermentation is conducted for 100 hours. When the concentration of glucose drops to 0.5%, the fermentation endpoint is achieved. The fermentation liquid containing erythritol is obtained, and the conversion rate of erythritol is 43.3%, which contains 135 grams of erythritol per liter.